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KMID : 0378019650080080059
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New Medical Journal
1965 Volume.8 No. 8 p.59 ~ p.77
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A Study of the Histological Changes and Growth Inhibition of the Chick Embryos After Incubation with Radioactive Phosphorus(P^32)
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Abstract
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The changes in histopathology and growth inhibition of the chick embryos incubated with P32 were experimentally studied. The various doses of P32 were injected into the yolk sac of the fertilized eggs of the white leghorn strain at the different intervals. The growth inhibition rates were evaluated on the basis of ,the embryos´ weight changes. On the other hand, the embryos killed on the 18th incubation day were used for tie study of the histopathological changes in the organs such as the gonads, bones, thymus, eye, thyroid and ´spleen. Following were the results:
1. The weight of the non-treated control group increased in parallel with the incubation days, particularly, a remarkable increase was noted after the ´12th incubation day.
L. The weight changes of the P32-treated groups were as follows:
a. Embryos treated on the 5th incubation day;
No weight differences were noted between the groups to which 5 and 10 pc of P32 were given on the 8th incubation day, however, the groups to which 25 and 50 pc were given showed the decreases of 9.1 and 13.6 % respectively. The growth inhibition rates of the groups to which 5, 10, 25 and 50 pc were given on the 12, 15 and 18 th incubation days were; 1.2, 2.4, 29.7 and 41.6 / ; 0.6, 1.7, 14.4 and $9.3% and 4.5, 7.4, 16.8 and 24.6 % respectively.
b. Embryos treated on the 8th incubation day;
The growth inhibition rates of the groups to which 5, 10, 25, 50 and 100 pc on the 12, 15 and 18th incubation days were; 1.2, 3.5, 34.5, 42.8 and 44.0 % ; 20.2, 24.8, 31.2, 39.9 and 38.1 % ; 20.5, 25.3, 28.7, 31.7 and 30.6 %, respectively. Thus, no notable difference was noted in the groups to which 50 and 100 pc were given.
c. Embryos treated on the 12th incubation day;
The growth inhibition rates of the groups to which 5, 10, 25, 50 and 100 pc on the 15 and 18 th Incubation days were; 2.9, 7.5, 26.6, 39.9, and 42.2 / ; 10.4, 14.2, 23.9, 30.0 and 32.8 % respectively.
5. The histopathological changes in the various organs sere as follows.
a. The testis and ovary were very sensitive to P32 especially the germ cells. The longer the incubation days the less the sensitivity and the larger the doses, the more pronounced the damage.
b. The bone tissues generally showed the reversible changes in the smaller doses. The cessation of mitosis, edematous degeneration and the decreased hyaline subtances were the mosth predominant changes.
c. The thymus appeared to be also very ´sensitive to P32. In larger doses, cell deformation, decreased leucocytes, deformation of the interlobular ´septa, hyperplasia of the connective tissues and the decreased Hassall´s body as well as the compensatroy hyperplasia were noted.
d. The ´eye showed no. notable changes in . smaller doses, however, the larger doses induced the severe changes such as the demarcation of the prechoroid tissue between the sclera and choroid, membrane, hemorrhage, infiltration of the leucocytes and the distortion of the retinal layers.
e. The thyroid gland showed the destruction of follicles, hemorrhage and hyperplasia of the interstitial tissues in the larger doses, however,the reversible phenomena were also observed.
f. The spleen showed the red cell destruction in the red pulp, decreased blood cells and hypertrophy of the trabeculae-in the larger doses.
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